Primary chronic osteomyelitis of the jaw—a microbial investigation using cultivation and DNA analysis: a pilot study
Presented at the Scandinavian Association of Oral and Maxillofacial Surgeons meeting, June 2008.
Received 4 July 2008; received in revised form 8 November 2008; accepted 5 January 2009.
Objectives
The aims of this study were to investigate the infectious etiology of primary chronic osteomyelitis (PCO) of the jaw using an extraoral sampling approach and molecular methods.
Study design
In this pilot study, bone biopsies from the mandibles of 5 patients with PCO were sampled with an extraoral sterile approach. Cultivation and polymerase chain reaction (PCR) were performed.
Results
Two of the biopsies yielded growth of Propionebacterium acnes. One biopsy also demonstrated Staphylococcus capitis. The biopsies with bacterial growth were also positive for the same bacteria by PCR analysis.
Conclusion
No specific bacterial etiology was demonstrated. However, the results of bacterial cultivation and PCR analysis were in agreement. Based on these findings, the molecular procedure used in this study can be considered to be suitable for identification of bacterial specimens in mandibular biopsies. Further studies are needed with larger patient populations to confirm these findings.
aSpecialist in Oral and Maxillofacial Surgery, Department of Oral and Maxillofacial Surgery, Haukeland University Hospital and Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway
bProfessor, Department of Oral and Maxillofacial Surgery, Haukeland University Hospital and Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway
cMedical Laboratory Technologist, Gade Institute—Oral Microbiology, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway
dProfessor, Gade Institute—Oral Microbiology, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway
Reprint requests: Paula Frid, Department of Oral and Maxillofacial Surgery, Ålesund County Hospital, 6026 Ålesund, Norway
Supported by the University of Bergen and the Scandinavian Association of Oral and Maxillofacial Surgeons.
ABSTRACT