Inhibition of Candida albicans biofilm formation on denture material
Abstract presented at the American Association for Dental Research Annual session, Orlando, Florida, March 6, 2006.
Received 2 September 2008; received in revised form 24 December 2008; accepted 7 January 2009.
Objective
The aim was to determine the ability of several thin-film polymer formulations, with and without incorporated antifungals, to inhibit Candida albicans biofilm growth on denture material. The inhibition of C. albicans biofilms on maxillary dentures could play a significant role in preventing the development of denture stomatitis.
Study design
Low-porosity and high-porosity thin-film polymer formulations were used and one of the following fungicides was added: 1) chlorhexidine diacetate at 1.0%; 2) nystatin at 1.0%; or 3) amphotericin B at 0.1%. These coatings were placed on rectangular (12 × 10 mm) dental resin material samples. A subset of the coated dental materials were brushed to simulate denture cleaning for 1 minute per day for 1 year. Candida albicans biofilms were formed on polymethylmethacrylate (PMMA) specimens placed in 24-well polystyrene plates, and the extent of biofilm formation on coated and noncoated specimens was assessed using a 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay.
Results
Thin-film polymer PMMA coatings alone, without an antifungal agent, produced a small significant reduction in C. albicans biofilm formation compared with control PMMA. However, incorporation of antifungal medications into the thin-film polymer reduced biofilm formation between 70% and 80% with nystatin, and between 50% and 60% with amphotericin B. Biofilm reduction with chlorhexidine (up to 98%) was significantly greater than all other formulations tested (P < .025).
Conclusion
This novel thin-film coating with various antifungals effectively inhibits C. albicans biofilm formation and should be evaluated as a potential preventive therapy for denture stomatitis.
aProfessor, Department of Dental Diagnostic Science, University of Texas Health Science Center at San Antonio, San Antonio, Texas
bBiomedical Development Corporation, San Antonio, Texas
cProfessor, Department of Restorative Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, Texas
dAssistant Professor, Department of Dental Diagnostic Science, University of Texas Health Science Center at San Antonio, San Antonio, Texas
eProfessor, Department of Biology, University of Texas at San Antonio, San Antonio, Texas
Reprint requests: Spencer W. Redding, DDS, MEd, Department of Dental Diagnostic Science, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900
Supported by an International Association for Dental Research/Glaxo Smith Kline Innovation in Oral Care Award in 2004 to the University of Texas Health Science Center at San Antonio and Biomedical Development Corporation.
1 B. Bhatt is a former employee and G. Siegel is a current employee and officer of Biomedical Development Corporation
2 S. Redding and R. Rawls have participated as investigators on several Small Business Innovation Research grants to Biomedical Development Corporation.
ABSTRACT