The effects of osteoformin on mineralisation and quality of newly formed bone during mandibular distraction osteogenesis in rabbits
Received 31 March 2009; received in revised form 10 July 2009; accepted 17 July 2009. published online 15 October 2009.
Objective
The aim of this experimental study was to evaluate the effects of osteoformin on mineralization and quality of newly formed bone during distraction osteogenesis in rabbits.
Study design
Distraction rate was as 1 mm/day for 5 days. In the experimental group, 100 μg/kg osteoformin in 100 μL/kg phosphate-buffered saline (PBS) was injected into the distraction area at day 1 and day 7 after surgery. In the control group, only 100 μL/kg PBS was injected on the same days. On day 42, the animals were killed.
Results
Bone mineral density and bone mineral content values increased significantly in the experimental group (P < .05). Newly formed bone areas, number of vessels and osteoblasts were significantly greater in experimental group (P < .05). The number of fibroblasts were also greater, but it was not significant statistically (P > .05). The number of osteoclasts was significantly lower in experimental group (P < .05).
Conclusion
The results of this study show that osteoformin improves healing of regenerate in distraction osteogenesis in rabbits.
aAssistant Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Karadeniz Technical University, Trabzon, Turkey
bAssistant Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Karadeniz Technical University, Trabzon, Turkey
cAssociate Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Karadeniz Technical University, Trabzon, Turkey
dResearch Assistant, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Karadeniz Technical University, Trabzon, Turkey
eAssociate Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Selçuk University, Konya, Turkey
fAssistant Professor, Faculty of Medicine, Department of Pathology, Karadeniz Technical University, Trabzon, Turkey
gProfessor, Faculty of Medicine, Department of Pathology, Selçuk University, Konya, Turkey
Reprint requests: A. Alper Pampu, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Karadeniz Technical University, Trabzon, Turkey
ABSTRACT