Bone healing in critical-size defects treated with platelet-rich plasma: a histologic and histometric study in the calvaria of diabetic rat
Received 6 May 2009; received in revised form 20 July 2009; accepted 6 August 2009. published online 18 November 2009.
Objective
The objective of this study was to histologically analyze the influence of the platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in the calvaria of diabetic rats.
Study design
A total of 20 diabetic rats were divided into 2 groups: C (control) and PRP. A 5-mm diameter CSD was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group PRP, 0.35 mL of PRP was placed in the defects. All animals were humanely killed 30 days postoperatively. Histometric and histologic analyses were performed. Data were statistically analyzed (t test, P < .05).
Results
No defect completely regenerated with bone. Group PRP had a statistically greater amount of bone formation than Group C (37.22% ± 6.00% and 21.68% ± 11.35%, respectively).
Conclusion
PRP placed in the defects significantly enhanced bone healing in CSD in the calvaria of diabetic rats both qualitatively and quantitatively.
aDivision of Bucco-Maxillo-Facial Surgery and Traumatology, Department of Clinic and Surgery, Dental School, Federal University of Alfenas – UNIFAL, Alfenas, Brazil
bDivision of Integrated Clinic, Dental School of Lavras, Lavras University Center – UNILAVRAS, Lavras, Brazil
cDivision of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University – UNESP, Araçatuba, Brazil
dDepartment of Clinical and Toxicological Analysis, Federal University of Alfenas – UNIFAL, Alfenas, Brazil
Reprint requests: Michel Reis Messora, DDS, PhD, Centro Universitário de Lavras - UNILAVRAS, Rua Padre José Poggel 506, CEP: 37200-000 Lavras, MG, Brazil
ABSTRACT