Attachment of cultured fibroblasts and ultrastructural analysis of simulated cervical resorptions treated with high-power lasers and MTA
Received 18 June 2009; received in revised form 26 August 2009; accepted 9 September 2009. published online 07 December 2009.
Objective
The present study evaluated the fibroblast attachment and the morphologic changes of simulated cervical root resorptions after irradiation with high-power lasers and the use of mineral trioxide aggregate (MTA).
Study design
Standardized cavities were done on the coronal third of 40 single-rooted teeth and assigned into 4 groups according to the treatment applied: (1) untreated control, (2) use of MTA, (3) irradiation with Er:YAG laser (42mJ, 10 Hz, 10 s), and (4) irradiation with high-power diode laser (1 W, 10 s). Fragments were prepared for SEM analysis: 6 samples of each group were plated with human gingival fibroblasts and 4 samples were used for ultrastructural analysis.
Results
The cells' attachment was in decreasing order: G3 > G4 > G1> G2, with statistical differences among all groups (P < .05). The samples irradiated with Er:YAG exhibited roughness on the dentinal surface, no smear layer, and open dentinal tubules; those irradiated with diode laser exhibited a smooth surface, more smear layer, and closed dentinal tubules; and the control group showed high amount of smear layer and closed dentin tubules.
Conclusions
Irradiation with Er:YAG and diode lasers caused morphologic changes on the dentinal surfaces of simulated resorptions that favored cells' adhesion. MTA showed lower biocompatibility than irradiated groups but allowed cells' adhesion.
aAssistant Professor, Division of Endodontics, Department of Dentistry, University of Taubate, Taubate, Sao Paulo, Brazil
bAssistant Professor, Division of Endodontology, Department of Restorative Dentistry University of Manitoba, Winnipeg, Manitoba, Canada
cAssistant Professor, Division of Prosthodontics, Department of Dentistry, University of Taubate, Taubate, Sao Paulo, Brazil
dCollaborator, Division of Endodontics, Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil
eChairman, Division of Endodontics, Department of Dentistry, University of Taubate, Taubate, Sao Paulo, Brazil
Reprint requests: Denise Pontes Raldi, DDS, MSc, PhD, Rua Expedicionário Ernesto Pereira 110, Taubate, São Paulo, Brazil, 12020-340
ABSTRACT